Summary

This report contains visualisations of statistics that can help in understanding the results from the wf-alignment workflow. Each section contains different plots or tables, and in general the results are broken down by sample or the reference file to which alignments were made. You can quickly jump to an individual section with the links in the header bar.

1 sample:
barcode95

1 reference file:
reference.txt

1 reference sequence:
KU168830.1

Metric Value Percentage
Detected reference sequences 1
100.0%
Reads 5,061
100.0%
Reads aligned to 'reference.txt' 1,513
29.9%
Unmapped reads 3,548
70.1%
Bases 36,553,998
100.0%
Metric Value Percentage
Detected reference sequences 1
100.0%
Reads 5,061
100.0%
Reads aligned to 'reference.txt' 1,513
29.9%
Unmapped reads 3,548
70.1%
Bases 36,553,998
100.0%

Read and alignment summary statistics

Read quality and length in addition to alignment accuracy and coverage are illustrated in the plots below.

Depth of coverage

This section illustrates the depth of coverage of the reference genomes. The left plot shows coverage vs. genomic position (note that the coordinates on the x-axis are the positions along the concatenated reference including all reference sequences in the respective reference file). The right plot shows the cumulative fraction of the reference that was covered to at least a certain depth.

Software versions

Name Version
python 3.8.18
seqkit v2.6.1
minimap2 2.26-r1175
samtools 1.18
fastcat 0.15.1
mosdepth 0.3.6
ezcharts 0.7.6
pysam 0.21.0
bgzip (htslib) 1.18

Workflow parameters

Key Value
out_dir /mnt/c/Users/Слепушонки/epi2melabs/instances/wf-alignment_01JPFSJ3GMM1D01MT9JPV7144H/output
fastq /mnt/d/Drosera/filtered_q10/barcode95
bam None
references /mnt/d/Drosera/reference
igv True
reference_mmi_file None
counts None
prefix None
sample None
sample_sheet None
depth_coverage True
analyse_unclassified False
minimap_preset dna
minimap_args None
threads 4
per_read_stats False